Spatial transcriptomics reveals unique gene expression changes in different brain regions after sleep deprivation.

ABSTRACT: Sleep deprivation has far-reaching consequences on the brain and behavior, impacting memory, attention, and metabolism. Previous research has focused on gene expression changes in individual brain regions, such as the hippocampus or cortex. Therefore, it is unclear how uniformly or heterogeneously sleep loss affects the brain. Here, we use spatial transcriptomics to define the impact of a brief period of sleep deprivation across the brain in male mice. We find that sleep deprivation induced pronounced differences in gene expression across the brain, with the greatest changes in the hippocampus, neocortex, hypothalamus, and thalamus. Both the differentially expressed genes and the direction of regulation differed markedly across regions. Importantly, we developed bioinformatic tools to regi

SUBMITTER: Vanrobaeys Y 

PROVIDER: S-EPMC10625558 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature