Chromatin accessibility profiling of targeted cell populations with laser capture microdissection coupled to ATAC-seq.

ABSTRACT: Spatially resolved omics technologies reveal context-dependent cellular regulatory networks in tissues of interest. Beyond transcriptome analysis, information on epigenetic traits and chromatin accessibility can provide further insights on gene regulation in health and disease. Nevertheless, compared to the enormous advancements in spatial transcriptomics technologies, the field of spatial epigenomics is much younger and still underexplored. In this study, we report laser capture microdissection coupled to ATAC-seq (LCM-ATAC-seq) applied to fresh frozen samples for the spatial characterization of chromatin accessibility. We first demonstrate the efficient use of LCM coupled to in situ tagmentation and evaluate its performance as a function of cell number, microdissected areas, and tissue t

SUBMITTER: Carraro C 

PROVIDER: S-EPMC10626193 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature