Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets.

ABSTRACT: First-phase glucose-stimulated insulin secretion is mechanistically linked to type 2 diabetes, yet the underlying metabolism is difficult to discern due to significant islet-to-islet variability. Here, we miniaturize a fluorescence anisotropy immunoassay onto a microfluidic device to measure C-peptide secretion from individual islets as a surrogate for insulin (InsC-chip). This method measures secretion from up to four islets at a time with ∼7 s resolution while providing an optical window for real-time live-cell imaging. Using the InsC-chip, we reveal two glucose-dependent peaks of insulin secretion (i.e., a double peak) within the classically defined 1^st phase (<10 min). By combining real-time secretion and live-cell imaging, we show islets transition from glycolytic to oxidat

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10626205 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature