PRELP secreted from mural cells protects the function of blood brain barrier through regulation of endothelial cell-cell integrity.

ABSTRACT: Introduction: Proline/arginine-rich end leucine-rich repeat protein (PRELP), is a small secreted proteoglycan expressed by pericytes and vascular smooth muscle cells surrounding the brain vasculature of adult mouse. Methods: We utilised a Prelp knockout (Prelp ^-/-) mouse model to interrogate vasculature integrity in the brain alongside performing in vitro assays to characterise PRELP application to endothelial cells lines. Our findings were supplemented with RNA expression profiling to elucidate the mechanism of how PRELP maintains neurovasculature function. Results: Prelp ^-/- mice presented with neuroinflammation and reducedneurovasculature integrity, resulting in IgG and dextran leakage in the cerebellum and cortex. Histolog

SUBMITTER: Davaapil H 

PROVIDER: S-EPMC10626469 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature