Citrate Phosphate Dextrose Alters Coagulation Dynamics Ex Vivo.

ABSTRACT:

Introduction

Citrate-phosphate-dextrose (CPD) is the most common anticoagulant for blood product storage in the United States. It was developed to prolong shelf life, though there is little research regarding its impact on function following transfusion. We used flow cytometry (FC), thromboelastography (TEG), and a clot contraction assay called the zFlex platform to measure platelet activation and global clot formation in blood samples anticoagulated with either CPD or in a standard blue top citrate (BTC) tube.

Methods

Samples were obtained through venipuncture of the antecubital fossa from healthy donors who had not recently taken antiplatelet medication. Samples for FC analysis were spun to obtain platelet-rich plasma, while TEG and zFlex utilized recalcified whole blood.<

SUBMITTER: Schriner JB 

PROVIDER: S-EPMC10626577 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature