Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction.

ABSTRACT:

Background

Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo development. In some mammals, like pigs, ejaculates are emitted in three separate fractions: pre-sperm, sperm-rich (SRF) and post sperm-rich (PSRF). These fractions are known to vary in volume, sperm concentration and quality, as well as in the origin and composition of seminal plasma (SP), with differences being also observed within the SRF one. Yet, whether disparities in the DNA integrity and chromatin condensation and protamination of their sperm exist has not been interrogated.

Results

This study determined chromatin protamination (Chromomycin A3 test, CMA₃), condensation (Dibromobimane test, DBB), and DNA integrity (Com

SUBMITTER: Vinolas-Verges E 

PROVIDER: S-EPMC10626759 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature