¹H, ¹⁵N, ¹³C backbone and sidechain resonance assignments and secondary structure of mouse NOTCH1 EGF27.

ABSTRACT: NOTCH1 is a transmembrane receptor in metazoans that is linked to a variety of disorders. The receptor contains an extracellular domain (ECD) with 36 tandem epidermal growth factor-like (EGF) repeats. The ECD is responsible for intercellular signaling via protein-ligand interactions with neighboring cells. Each EGF repeat consists of approximately 40 amino acids and 3 conserved disulfide bonds. The Abruptex region (EGF24-29) is critical for NOTCH1 signaling and is known for its missense mutations. Certain EGF repeats are modified with the addition of O-linked glycans and many have calcium binding sites, which give each EGF repeat a unique function. It has been shown that the loss of the O-fucose site of EGF27 alters NOTCH1 activity. To investigate the role of glycosylation in the NOTCH1 si

SUBMITTER: Grennell JA 

PROVIDER: S-EPMC10626972 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature