CD14^highCD16⁺ monocytes are the main producers of Interleukin-10 following clinical heart transplantation.

ABSTRACT:

Introduction

Following heart transplantation, a cascade of immunological responses is initiated influencing the clinical outcome and long-term survival of the transplanted patients. The anti-inflammatory cytokine interleukin-10 (IL-10) was shown to be elevated in the blood of heart transplant recipients directly after transplantation but the releasing cell populations and the composition of lymphocyte subsets following transplantation have not been thoroughly studied.

Methods

We identified immune cells by immunophenotyping and analyzed intracellular IL-10 production in peripheral blood mononuclear cells (PBMC) of heart transplanted patients (n= 17) before, directly after and 24h post heart transplantation. The cells were stimulated with lipopolysaccharide or PMA/Ionomycin to

SUBMITTER: Ludwig K 

PROVIDER: S-EPMC10627027 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature