A photoaffinity glycan-labeling approach to investigate immunoglobulin glycan-binding partners.

ABSTRACT: Glycans play a pivotal role in biology. However, because of the low-affinity of glycan-protein interactions, many interaction pairs remain unknown. Two important glycoproteins involved in B-cell biology are the B-cell receptor and its secreted counterpart, antibodies. It has been indicated that glycans expressed by these B-cell-specific molecules can modulate immune activation via glycan-binding proteins. In several autoimmune diseases, an increased prevalence of variable domain glycosylation of IgG autoantibodies has been observed. Especially, the hallmarking autoantibodies in rheumatoid arthritis, anti-citrullinated protein antibodies, carry a substantial amount of variable domain glycans. The variable domain glycans expressed by these autoantibodies are N-linked, complex-type, and α2-6

SUBMITTER: Holborough-Kerkvliet MD 

PROVIDER: S-EPMC10627247 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature