SynLight: a bicistronic strategy for simultaneous active zone and cell labeling in the Drosophila nervous system.

ABSTRACT: At synapses, chemical neurotransmission mediates the exchange of information between neurons, leading to complex movement, behaviors, and stimulus processing. The immense number and variety of neurons within the nervous system make discerning individual neuron populations difficult, necessitating the development of advanced neuronal labeling techniques. In Drosophila, Bruchpilot-Short and mCD8-GFP, which label presynaptic active zones and neuronal membranes, respectively, have been widely used to study synapse development and organization. This labeling is often achieved via the expression of 2 independent constructs by a single binary expression system, but expression can weaken when multiple transgenes are expressed by a single driver. Recent work has sought to circumvent these drawbacks

SUBMITTER: Aimino MA 

PROVIDER: S-EPMC10627267 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature