A community resource to mass explore the wheat grain proteome and its application to the late-maturity alpha-amylase (LMA) problem.

ABSTRACT:

Background

Late-maturity alpha-amylase (LMA) is a wheat genetic defect causing the synthesis of high isoelectric point alpha-amylase following a temperature shock during mid-grain development or prolonged cold throughout grain development, both leading to starch degradation. While the physiology is well understood, the biochemical mechanisms involved in grain LMA response remain unclear. We have applied high-throughput proteomics to 4,061 wheat flours displaying a range of LMA activities. Using an array of statistical analyses to select LMA-responsive biomarkers, we have mined them using a suite of tools applicable to wheat proteins.

Results

We observed that LMA-affected grains activated their primary metabolisms such as glycolysis and gluconeogenesis; TCA cycle, along with

SUBMITTER: Vincent D 

PROVIDER: S-EPMC10627334 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature