Evidence of Site-Specific and Male-Biased Germline Mutation Rate in a Wild Songbird.

ABSTRACT: Germline mutations are the ultimate source of genetic variation and the raw material for organismal evolution. Despite their significance, the frequency and genomic locations of mutations, as well as potential sex bias, are yet to be widely investigated in most species. To address these gaps, we conducted whole-genome sequencing of 12 great reed warblers (Acrocephalus arundinaceus) in a pedigree spanning 3 generations to identify single-nucleotide de novo mutations (DNMs) and estimate the germline mutation rate. We detected 82 DNMs within the pedigree, primarily enriched at CpG sites but otherwise randomly located along the chromosomes. Furthermore, we observed a pronounced sex bias in DNM occurrence, with male warblers exhibiting three times more mutations than females. After correction f

SUBMITTER: Zhang H 

PROVIDER: S-EPMC10627410 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature