Pulse train gating to improve signal generation for in vivo two-photon fluorescence microscopy.

ABSTRACT:

Significance

Two-photon microscopy is used routinely for in vivo imaging of neural and vascular structures and functions in rodents with a high resolution. Image quality, however, often degrades in deeper portions of the cerebral cortex. Strategies to improve deep imaging are therefore needed. We introduce such a strategy using the gating of high repetition rate ultrafast pulse trains to increase the signal level.

Aim

We investigate how the signal generation, signal-to-noise ratio (SNR), and signal-to-background ratio (SBR) improve with pulse gating while imaging in vivo mouse cerebral vasculature.

Approach

An electro-optic modulator with a high-power (6 W) 80 MHz repetition rate ytterbium fiber amplifier is used to create gates of pulses at a 1 MHz repe

SUBMITTER: Engelmann SA 

PROVIDER: S-EPMC10627479 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature