Enucleation of the C. elegans embryo revealed dynein-dependent spacing between microtubule asters.

ABSTRACT: The intracellular positioning of the centrosome, a major microtubule-organizing center, is important for cellular functions. One of the features of centrosome positioning is the spacing between centrosomes; however, the underlying mechanisms are not fully understood. To characterize the spacing activity in Caenorhabditis elegans embryos, a genetic setup was developed to produce enucleated embryos. The centrosome was duplicated multiple times in the enucleated embryo, which enabled us to characterize the chromosome-independent spacing activity between sister and non-sister centrosome pairs. We found that the timely spacing depended on cytoplasmic dynein, and we propose a stoichiometric model of cortical and cytoplasmic pulling forces for the spacing between centrosomes. We also obser

SUBMITTER: Fujii K 

PROVIDER: S-EPMC10627822 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature