Comparison of loop-mediated isothermal amplification, multiplex PCR, and REP- PCR techniques for identification of carbapenem-resistant Acinetobacter baumannii clinical isolates.

ABSTRACT:

Background and objectives

Acinetobacter baumannii, an opportunistic pathogen, is related to hospital-acquired infections and increased mortality. This study aimed to develop the loop-mediated isothermal amplification (LAMP) test for the fast-detecting of A. baumannii isolates as well as determining genetic relatedness for these isolates via the REP-PCR technique.

Materials and methods

LAMP primers and multiplex PCR primers were designed for recognizing A. baumannii isolates harboring the bla _SHV-1 , bla _PER-1 , bla _TEM-1, AMPC, qnr, and aac (6)-1 genes, were collected (October 2020 to February 2021) from Shahid Motahari Hospital, Tehran, Iran. Combination disc test (CDT) results were use

SUBMITTER: Al Jebur AA 

PROVIDER: S-EPMC10628086 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature