⁸⁹Zr-leukocyte labelling for cell trafficking: in vitro and preclinical investigations.

ABSTRACT:

Background

The non-invasive imaging of leukocyte trafficking to assess inflammatory areas and monitor immunotherapy is currently generating great interest. There is a need to develop more robust cell labelling and imaging approaches to track living cells. Positron emission tomography (PET), a highly sensitive molecular imaging technique, allows precise signals to be produced from radiolabelled moieties. Here, we developed a novel leukocyte labelling approach with the PET radioisotope zirconium-89 (⁸⁹Zr, half-life of 78.4 h). Experiments were carried out using human leukocytes, freshly isolated from whole human blood.

Results

The ⁸⁹Zr-leukocyte labelling efficiency ranged from 46 to 87% after 30-60 min. Radioactivity concentrations of labelled cells were

SUBMITTER: Kahts M 

PROVIDER: S-EPMC10628102 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature