Three-dimensional label-free morphology of CD8 + T cells as a sepsis biomarker.

ABSTRACT: Sepsis is a dysregulated immune response to infection that leads to organ dysfunction and is associated with a high incidence and mortality rate. The lack of reliable biomarkers for diagnosing and prognosis of sepsis is a major challenge in its management. We aimed to investigate the potential of three-dimensional label-free CD8 + T cell morphology as a biomarker for sepsis. This study included three-time points in the sepsis recovery cohort (N = 8) and healthy controls (N = 20). Morphological features and spatial distribution within cells were compared among the patients' statuses. We developed a deep learning model to predict the diagnosis and prognosis of sepsis using the internal cell morphology. Correlation between the morphological features and clinical indices were analysed. Cell mo

SUBMITTER: Sung M 

PROVIDER: S-EPMC10628166 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature