Collagen-I influences the post-translational regulation, binding partners and role of Annexin A2 in breast cancer progression.

ABSTRACT:

Introduction

The extracellular matrix (ECM) has been heavily implicated in the development and progression of cancer. We have previously shown that Annexin A2 is integral in the migration and invasion of breast cancer cells and in the clinical progression of ER-negative breast cancer, processes which are highly influenced by the surrounding tumor microenvironment and ECM.

Methods

We investigated how modulations of the ECM may affect the role of Annexin A2 in MDA-MB-231 breast cancer cells using western blotting, immunofluorescent confocal microscopy and immuno-precipitation mass spectrometry techniques.

Results

We have shown that the presence of collagen-I, the main constituent of the ECM, increases the post-translational phosphorylation of Annexin A2 and subsequently

SUBMITTER: Mahdi AF 

PROVIDER: S-EPMC10628465 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature