CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signaling.

ABSTRACT:

Introduction

CXCL17 is a mucosally secreted protein, and the most recently identified human chemokine, an assignment based on protein fold prediction and chemotactic activity for leukocytes. However, these credentials have been the subject of much recent discussion and no experimental evidence has been presented regarding the definitive structure of CXCL17. In this study, we evaluated the structural and chemoattractant credentials of CXCL17 to better characterize this molecule, and gain deeper insights into its functional role as a glycosaminoglycan (GAG) binding protein.

Methods

In the absence of structural information, in silico modeling techniques assessed the likelihood of CXCL17 adopting a chemokine fold. Recombinant CXCL17 was synthesized in mammalian and prokar

SUBMITTER: Giblin SP 

PROVIDER: S-EPMC10628517 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature