Diverse clonal fates emerge upon drug treatment of homogeneous cancer cells.

ABSTRACT: Even among genetically identical cancer cells, resistance to therapy frequently emerges from a small subset of those cells^1-7. Molecular differences in rare individual cells in the initial population enable certain cells to become resistant to therapy^7-9; however, comparatively little is known about the variability in the resistance outcomes. Here we develop and apply FateMap, a framework that combines DNA barcoding with single-cell RNA sequencing, to reveal the fates of hundreds of thousands of clones exposed to anti-cancer therapies. We show that resistant clones emerging from single-cell-derived cancer cells adopt molecularly, morphologically and functionally distinct resistant types. These resistant types are largely predetermined by molecular differences between

SUBMITTER: Goyal Y 

PROVIDER: S-EPMC10628994 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature