Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplification.

ABSTRACT:

Background

Goose astrovirus (GoAstV) is an important pathogen that causes joint and visceral gout in goslings. It has been circulating in many provinces of China since 2017. Goose astrovirus genotypes 2 (GoAstV-2) is the main epidemic strain, and its high morbidity and mortality have caused huge economic losses to the goose industry. An accurate point-of-care detection for GoAstV-2 is of great significance. In this study, we developed a real-time reverse transcription recombinase polymerase amplification (RT-RPA) method for the on-site detection of GoAstV-2 infection.

Results

The real-time RT-RPA reaction was carried out at a constant temperature of 39 °C, and the entire detection time from nucleic acid preparation to the end of amplification was only 25 min using the portab

SUBMITTER: Li H 

PROVIDER: S-EPMC10629041 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature