Safety and efficacy of human ESC-derived corneal endothelial cells for corneal endothelial dysfunction.

ABSTRACT:

Background

Research on human pluripotent stem cells (hPSCs) has shown tremendous progress in cell-based regenerative medicine. Corneal endothelial dysfunction is associated with the loss and degeneration of corneal endothelial cells (CECs), rendering cell replacement a promising therapeutic strategy. However, comprehensive preclinical assessments of hPSC-derived CECs for this cell therapy remain a challenge.

Results

Here we defined an adapted differentiation protocol to generate induced corneal endothelial cells (iCECs) consistently and efficiently from clinical-grade human embryonic stem cells (hESCs) with xeno-free medium and manufactured cryopreserved iCECs. Cells express high levels of typical CECs markers and exhibit transendothelial potential properties in vitro typica

SUBMITTER: Yu J 

PROVIDER: S-EPMC10629087 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature