Modulation of protein-DNA binding reveals mechanisms of spatiotemporal gene control in early Drosophila embryos.

ABSTRACT: It is well known that enhancers regulate the spatiotemporal expression of their target genes by recruiting transcription factors (TFs) to the cognate binding sites in the region. However, the role of multiple binding sites for the same TFs and their specific spatial arrangement in determining the overall competency of the enhancer has yet to be fully understood. In this study, we utilized the MS2-MCP live imaging technique to quantitatively analyze the regulatory logic of the snail distal enhancer in early Drosophila embryos. Through systematic modulation of Dorsal and Twist binding motifs in this enhancer, we found that a mutation in any one of these binding sites causes a drastic reduction in transcriptional amplitude, resulting in a reduction in mRNA production of the targ

SUBMITTER: Syed S 

PROVIDER: S-EPMC10629816 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature