Ontology highlight
ABSTRACT:
SUBMITTER: Shaw J
PROVIDER: S-EPMC10630134 | biostudies-literature | 2023 Nov
REPOSITORIES: biostudies-literature
Nature methods 20230921 11
Sequence comparison tools for metagenome-assembled genomes (MAGs) struggle with high-volume or low-quality data. We present skani ( https://github.com/bluenote-1577/skani ), a method for determining average nucleotide identity (ANI) via sparse approximate alignments. skani outperforms FastANI in accuracy and speed (>20× faster) for fragmented, incomplete MAGs. skani can query genomes against >65,000 prokaryotic genomes in seconds and 6 GB memory. skani unlocks higher-resolution insights for exte ...[more]