UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine.

ABSTRACT: Ultraviolet irradiation is an effective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fluorescent GFP protein produced by baculovirus expression system after the irradiation of baculovirus culture in doses ranging from 3.5 to 42 J/m² was determined. At a dose of 36.8 J/m², only 0.5% of GFP-expressing cells were detected by flow cytometry and confocal microscopy. The stability of purified VP1-PCV2bCap protein produced by baculovirus expression system was analyzed after the irradiation at doses ranging from 3.5 to 19.3 J/m². Up to the dose of 11 J/m², no significant effect of UV-C light on the stability of VP1-PCV2bCap was detected. We observed a dose-dependent increase in

SUBMITTER: Vrablikova A 

PROVIDER: S-EPMC10630496 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature