Genetically Encoded RNA-Based Bioluminescence Resonance Energy Transfer (BRET) Sensors.

ABSTRACT: RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce output signals, most often a fluorescence readout. Although powerful, the high reliance of fluorescence on the external excitation light raises concerns about its high background, photobleaching, and phototoxicity. Bioluminescence signals can be an ideal complementary readout for these genetically encoded RNA nanodevices. However, RNA-based real-time bioluminescent reporters have been rarely developed. In this study, we reported the first type of genetically encoded RNA-based bioluminescence resonance energy transfer (BRET) sensors that ca

SUBMITTER: Mi L 

PROVIDER: S-EPMC10630924 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature