Comprehensive mapping of exon junction complex binding sites reveals universal EJC deposition in Drosophila.

ABSTRACT:

Background

The exon junction complex (EJC) is involved in most steps of the mRNA life cycle, ranging from splicing to nonsense-mediated mRNA decay (NMD). It is assembled by the splicing machinery onto mRNA in a sequence-independent manner. A fundamental open question is whether the EJC is deposited onto all exon‒exon junctions or only on a subset of them. Several previous studies have made observations supportive of the latter, yet these have been limited by methodological constraints.

Results

In this study, we sought to overcome these limitations via the integration of two different approaches for transcriptome-wide mapping of EJCs. Our results revealed that nearly all, if not all, internal exons consistently harbor an EJC in Drosophila, demonstrating that EJC presence is a

SUBMITTER: Morillo L 

PROVIDER: S-EPMC10630996 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature