Impact of library input on the hit discovery rate in DNA-encoded chemical library selections.
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ABSTRACT:
SUBMITTER: Puglioli S
PROVIDER: S-EPMC10631129 | biostudies-literature | 2023 Nov
REPOSITORIES: biostudies-literature
Publications
Impact of library input on the hit discovery rate in DNA-encoded chemical library selections.
Puglioli Sara S Oehler Sebastian S Prati Luca L Scheuermann Jörg J Bassi Gabriele G Cazzamalli Samuele S Neri Dario D Favalli Nicholas N
Chemical science 20231017 43
DNA-encoded chemical libraries (DELs) are powerful drug discovery tools, enabling the parallel screening of millions of DNA-barcoded compounds. We investigated how the DEL input affects the hit discovery rate in DEL screenings. Evaluation of selection fingerprints revealed that the use of approximately 10<sup>5</sup> copies of each library member is required for the confident identification of nanomolar hits, using generally applicable methodologies. ...[more]
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