Impact of library input on the hit discovery rate in DNA-encoded chemical library selections.

ABSTRACT: DNA-encoded chemical libraries (DELs) are powerful drug discovery tools, enabling the parallel screening of millions of DNA-barcoded compounds. We investigated how the DEL input affects the hit discovery rate in DEL screenings. Evaluation of selection fingerprints revealed that the use of approximately 105 copies of each library member is required for the confident identification of nanomolar hits, using generally applicable methodologies.

SUBMITTER: Puglioli S 

PROVIDER: S-EPMC10631129 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature