Polarized macrophages regulate fibro/adipogenic progenitor (FAP) adipogenesis through exosomes.

ABSTRACT:

Background

Macrophage polarization has been observed in the process of muscle injuries including rotator cuff (RC) muscle atrophy and fatty infiltration after large tendon tears. In our previous study, we showed that fibrogenesis and white adipogenesis of muscle residential fibro/adipogenic progenitors (FAPs) cause fibrosis and fatty infiltration and that brown/beige adipogenesis of FAPs promotes rotator cuff muscle regeneration. However, how polarized macrophages and their exosomes regulate FAP differentiation remains unknown.

Methods

We cultured FAPs with M0, M1, and M2 macrophages or 2 × 10⁹ exosomes derived from M0, M1 and M2 with and without GW4869, an exosome inhibitor. In vivo, M0, M1, and M2 macrophages were transplanted or purified macrophage exosomes (M0

SUBMITTER: Liu M 

PROVIDER: S-EPMC10631219 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature