Nestin Regulates Muller Glia Proliferation After Retinal Injury.

ABSTRACT:

Purpose

The proliferative and neurogenic potential of retinal Müller glia after injury varies widely across species. To identify the endogenous mechanisms regulating the proliferative response of mammalian Müller glia, we comparatively analyzed the expression and function of nestin, an intermediate filament protein established as a neural stem cell marker, in the mouse and rat retinas after injury.

Methods

Nestin expression in the retinas of C57BL/6 mice and Wistar rats after methyl methanesulfonate (MMS)-induced photoreceptor injury was examined by immunofluorescence and Western blotting. Adeno-associated virus (AAV)-delivered control and nestin short hairpin RNA (shRNA) were intravitreally injected to rats and Müller glia proliferation after MMS-induced injury was analyzed

SUBMITTER: Motoyoshi A 

PROVIDER: S-EPMC10631512 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature