Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus.

ABSTRACT: The development and diversity of neuronal subtypes in the human hypothalamus has been insufficiently characterized. To address this, we integrated transcriptomic data from 241,096 cells (126,840 newly generated) in the prenatal and adult human hypothalamus to reveal a temporal trajectory from proliferative stem cell populations to mature hypothalamic cell types. Iterative clustering of the adult neurons identified 108 robust transcriptionally distinct neuronal subtypes representing 10 hypothalamic nuclei. Pseudotime trajectories provided insights into the genes driving formation of these nuclei. Comparisons to single-cell transcriptomic data from the mouse hypothalamus suggested extensive conservation of neuronal subtypes despite certain differences in species-enriched gene expression. The

SUBMITTER: Herb BR 

PROVIDER: S-EPMC10631741 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature