Mesenchymal Stromal Cell Secretome Is Affected by Tissue Source and Donor Age.

ABSTRACT: Variation in mesenchymal stromal cell (MSC) function depending on their origin is problematic, as it may confound clinical outcomes of MSC therapy. Current evidence suggests that the therapeutic benefits of MSCs are attributed to secretion of biologically active factors (secretome). However, the effect of donor characteristics on the MSC secretome remains largely unknown. Here, we examined the influence of donor age, sex, and tissue source, on the protein profile of the equine MSC secretome. We used dynamic metabolic labeling with stable isotopes combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify secreted proteins in MSC conditioned media (CM). Seventy proteins were classified as classically secreted based on the rate of label incorporation into newly synth

SUBMITTER: Turlo AJ 

PROVIDER: S-EPMC10631804 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature