Predictions of DNA mechanical properties at a genomic scale reveal potentially new functional roles of DNA flexibility.

ABSTRACT: Mechanical properties of DNA have been implied to influence many of its biological functions. Recently, a new high-throughput method, called loop-seq, which allows measuring the intrinsic bendability of DNA fragments, has been developed. Using loop-seq data, we created a deep learning model to explore the biological significance of local DNA flexibility in a range of different species from different kingdoms. Consistently, we observed a characteristic and largely dinucleotide-composition-driven change of local flexibility near transcription start sites. In the presence of a TATA-box, a pronounced peak of high flexibility can be observed. Furthermore, depending on the transcription factor investigated, flanking-sequence-dependent DNA flexibility was identified as a potential factor influenc

SUBMITTER: Back G 

PROVIDER: S-EPMC10632188 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature