Control of A/D type CpG-ODN aggregates to a suitable size for induction of strong immunostimulant activity.

ABSTRACT: Among several types of CpG-ODNs, A/D-type CpG-ODNs have potent adjuvant activity to induce Th-1 immune responses, but exhibit a propensity to aggregate. For the clinical application of A/D-type CpG-ODNs, it is necessary to control such aggregation and obtain a comprehensive understanding of the relationship between their structure and the immune responses. This study revealed that a representative A/D-type CpG ODN, D35, adopted a single-stranded structure in water, while it assembled into aggregates in response to Na⁺ ions. From polyacrylamide gel electrophoresis and circular dichroism analyses, D35 adopted a homodimeric form (duplex) via palindromic sequences in low-Na⁺-concentration conditions (10-50 mM NaCl). After replacement of the solution with PBS, quadruplexes

SUBMITTER: Matsuda M 

PROVIDER: S-EPMC10633530 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature