PINK1-PTEN axis promotes metastasis and chemoresistance in ovarian cancer via non-canonical pathway.

ABSTRACT:

Background

Ovarian cancer is commonly associated with a poor prognosis due to metastasis and chemoresistance. PINK1 (PTEN-induced kinase 1) is a serine/threonine kinase that plays a crucial part in regulating various physiological and pathophysiological processes in cancer cells.

Methods

The ATdb database and "CuratedOvarianData" were used to evaluate the effect of kinases on ovarian cancer survival. The gene expression in ovarian cancer cells was detected by Western blot and quantitative real-time PCR. The effects of gene knockdown or overexpression in vitro were evaluated by wound healing assay, cell transwell assay, immunofluorescence staining, immunohistochemistry, and flow cytometry analysis. Mass spectrometry analysis, protein structure analysis, co-immunoprecipitation

SUBMITTER: Zheng F 

PROVIDER: S-EPMC10633943 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature