Efferocytosis is restricted by axon guidance molecule EphA4 via ERK/Stat6/MERTK signaling following brain injury.

ABSTRACT:

Background

Efferocytosis is a process that removes apoptotic cells and cellular debris. Clearance of these cells alleviates neuroinflammation, prevents the release of inflammatory molecules, and promotes the production of anti-inflammatory cytokines to help maintain tissue homeostasis. The underlying mechanisms by which this occurs in the brain after injury remain ill-defined.

Methods

We used GFP bone marrow chimeric knockout (KO) mice to demonstrate that the axon guidance molecule EphA4 receptor tyrosine kinase is involved in suppressing MERTK in the brain to restrict efferocytosis of resident microglia and peripheral-derived monocyte/macrophages.

Results

Single-cell RNAseq identified MERTK expression, the primary receptor involved in efferocytosis, on monocytes, mic

SUBMITTER: Soliman E 

PROVIDER: S-EPMC10633953 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature