Phylogeny of the main bacterial 16S rRNA sequences in Drentse A grassland soils (The Netherlands).

ABSTRACT: The main bacteria in peaty, acid grassland soils in the Netherlands were investigated by ribosome isolation, temperature gradient gel electrophoresis, hybridization, cloning, and sequencing. Instead of using only 16S rDNA to determine the sequences present, we focused on rRNA to classify and quantify the most active bacteria. After direct ribosome isolation from soil, a partial amplicon of bacterial 16S rRNA was generated by reverse transcription-PCR. The sequence-specific separation by temperature gradient gel electrophoresis yielded soil-specific fingerprints, which were compared to signals from a clone library of genes coding for 16S rRNA. Cloned 16S rDNA sequences matching with intense bands in the fingerprint were sequenced. The relationships of the sequences to those of cultured orga

SUBMITTER: Felske A 

PROVIDER: S-EPMC106340 | biostudies-literature | 1998 Mar

REPOSITORIES: biostudies-literature