Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera).

ABSTRACT:

Objectives

DNA Barcoding has proven to be a reliable method for rapid insect identification. The success of this method is based on the amplification of a specific region, the 'Folmer' barcode region at the 5´ start of the cytochrome c oxidase 1 gene (cox1), with universal primers. Previous studies showed failures of standard "universal" primers to amplify this region in psyllids. The aim of the study was the design of a new alternative more reliable primer combination for taxa of the superfamily Psylloidea and its comparison with the performance of the standard "universal" Folmer-primers.

Results

A newly designed degenerate forward primer LCOP-F was developed following comparison of the sequence alignment of the priming site of "universal" primer LCO1490 and the standard in

SUBMITTER: Bastin S 

PROVIDER: S-EPMC10634070 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature