Changing turn-over rates regulate abundance of tryptophan, GS biosynthesis, IAA transport and photosynthesis proteins in Arabidopsis growth defense transitions.

ABSTRACT:

Background

Shifts in dynamic equilibria of the abundance of cellular molecules in plant-pathogen interactions need further exploration. We induced PTI in optimally growing Arabidopsis thaliana seedlings for 16 h, returning them to growth conditions for another 16 h.

Methods

Turn-over and abundance of 99 flg22 responding proteins were measured chronologically using a stable heavy nitrogen isotope partial labeling strategy and targeted liquid chromatography coupled to mass spectrometry (PRM LC-MS). These experiments were complemented by measurements of mRNA and phytohormone levels.

Results

Changes in synthesis and degradation rate constants (K_s and K_d) regulated tryptophane and glucosinolate, IAA transport, and photosynthesis-associated protein (PA

SUBMITTER: Abukhalaf M 

PROVIDER: S-EPMC10634109 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature