Transcriptomic and metabolomic analyses reveal the essential nature of Rab1B in Toxoplasma gondii.

ABSTRACT:

Background

The protozoan parasite Toxoplasma gondii encodes a dozen Rab proteins, which are parts of the small GTPase superfamily and regulate intracellular membrane trafficking. Our previous study showed that depletion of Rab1B caused severe defects regarding parasite growth and morphological structure, yet early defects of endocytic trafficking and vesicle sorting to the rhoptry in T. gondii are not expected to have a strong effect. To understand this discrepancy, we performed an integrated analysis at the level of transcriptomics and metabolomics.

Methods

In the study, tetracycline-inducible TATi/Ty-Rab1B parasite line treated with ATc at three different time points (0, 18 and 24 h) was used. We first observed the morphological changes caused by Rab1B depletion via transm

SUBMITTER: He K 

PROVIDER: S-EPMC10634116 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature