Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis.

ABSTRACT: Extracellular vesicles (EVs) in blood plasma are recognized as potential biomarkers for disease. Although blood plasma is easily obtainable, analysis of EVs at the single particle level is still challenging due to the biological complexity of this body fluid. Besides EVs, plasma contains different types of lipoproteins particles (LPPs), that outnumber EVs by orders of magnitude and which partially overlap in biophysical properties such as size, density and molecular makeup. Consequently, during EV isolation LPPs are often co-isolated. Furthermore, physical EV-LPP complexes have been observed in purified EV preparations. Since co-isolation or association of LPPs can impact EV-based analysis and biomarker profiling, we investigated the presence and formation of EV-LPP complexes in biological

SUBMITTER: Lozano-Andres E 

PROVIDER: S-EPMC10634195 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature