Characterization of transcriptional enhancers in the chicken genome using CRISPR-mediated activation.

ABSTRACT: DNA regulatory elements intricately control when, where, and how genes are activated. Therefore, understanding the function of these elements could unveil the complexity of the genetic regulation network. Genome-wide significant variants are predominantly found in non-coding regions of DNA, so comprehending the predicted functional regulatory elements is crucial for understanding the biological context of these genomic markers, which can be incorporated into breeding programs. The emergence of CRISPR technology has provided a powerful tool for studying non-coding regulatory elements in genomes. In this study, we leveraged epigenetic data from the Functional Annotation of Animal Genomes project to identify promoter and putative enhancer regions associated with three genes (HBBA, IRF7

SUBMITTER: Han JH 

PROVIDER: S-EPMC10634339 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature