Determining the impact of vaccination on SARS-CoV-2 RT-PCR cycle threshold values and infectious viral titres.

ABSTRACT:

Background

As the COVID-19 pandemic continues, efforts to better understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral shedding and transmission in both unvaccinated and vaccinated populations remain critical to informing public health policies and vaccine development. The utility of using real time RT-PCR cycle threshold values (C_T values) as a proxy for infectious viral litres from individuals infected with SARS-CoV-2 is yet to be fully understood. This retrospective observational cohort study compares quantitative infectious viral litres derived from a focus-forming viral titre assay with SARS-CoV-2 RT-PCR C_T values in both unvaccinated and vaccinated individuals infected with the Delta strain.

Methods

Nasopharyngeal swabs pos

SUBMITTER: Peterson KL 

PROVIDER: S-EPMC10634488 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature