Showing the limitations of available phenotypic assays to detect Burkholderia pseudomallei from clinical specimens in Nigeria.

ABSTRACT: The genus Burkholderia comprises Gram-negative bacteria that are metabolically complex and versatile, often thriving in hostile settings. Burkholderia pseudomallei , the causative agent of melioidosis, is a prominent member of the genus and a clinical pathogen in tropical and sub-tropical regions. This pathogen is well known for its multidrug resistance and possible bioweapon potential. There is currently no report of the pathogen from clinical specimens in Nigeria, which might be due to misdiagnosis with phenotypic assays. This study aims to explore the accuracy of the use of phenotypic assays to diagnose B. pseudomallei in Nigeria. Two hundred and seventeen clinical samples and 28 Gram-negative clinical isolates were collected and analysed using Ashdown's selective agar and monoclonal an

SUBMITTER: Orababa OQ 

PROVIDER: S-EPMC10634492 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature