Identification of prosthetic joint infection by Candida using metagenomic shotgun sequencing.

ABSTRACT: Periprosthetic joint infection (PJI) is a serious complication after total knee arthroplasty. Fungal infections are prone to biofilm formation, which makes it hard to diagnose and clarify the pathogenic species. This case study provides evidence of a novel PJI pathogen that is otherwise difficult to detect using conventional methods. A patient was reviewed with persistent postoperative pain, swelling and eventually drainage around the left knee after undergoing a bilateral total knee arthroplasty 2 years previously for progressive osteoarthritis. By using metagenomic shotgun sequencing to analyse both bacterial and fungal agent sequences, we were able to identify fungal strains of Candida tropicalis, a rarely reported and difficult-to-culture PJI pathogen. Metagenomic shotgun sequencing en

SUBMITTER: Lin L 

PROVIDER: S-EPMC10634495 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature