Comprehensive single cell aging atlas of mammary tissues reveals shared epigenomic and transcriptomic signatures of aging and cancer.

ABSTRACT: Aging is the greatest risk factor for breast cancer; however, how age-related cellular and molecular events impact cancer initiation is unknown. We investigate how aging rewires transcriptomic and epigenomic programs of mouse mammary glands at single cell resolution, yielding a comprehensive resource for aging and cancer biology. Aged epithelial cells exhibit epigenetic and transcriptional changes in metabolic, pro-inflammatory, or cancer-associated genes. Aged stromal cells downregulate fibroblast marker genes and upregulate markers of senescence and cancer-associated fibroblasts. Among immune cells, distinct T cell subsets (Gzmk⁺, memory CD4⁺, γδ) and M2-like macrophages expand with age. Spatial transcriptomics reveal co-localization of aged immune and epithe

SUBMITTER: Angarola BL 

PROVIDER: S-EPMC10634680 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature