Ultrasensitive and multiplexed protein imaging with clickable and cleavable fluorophores.

ABSTRACT: Single-cell spatial proteomic analysis holds great promise to advance our understanding of the composition, organization, interaction and function of the various cell types in complex biological systems. However, the current multiplexed protein imaging technologies suffer from low detection sensitivity, limited multiplexing capacity or technically demanding. To tackle these issues, here we report the development of a highly sensitive and multiplexed in situ protein profiling method using off-the-shelf antibodies. In this approach, the protein targets are stained with horseradish peroxidase (HRP) conjugated antibodies and cleavable fluorophores via click chemistry. Through reiterative cycles of target staining, fluorescence imaging, and fluoropohore cleavage, many proteins can be profiled i

SUBMITTER: Pham T 

PROVIDER: S-EPMC10634699 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature