Structure of the Thin Filament in Human iPSC-derived Cardiomyocytes and its Response to Heart Disease.

ABSTRACT: Cardiovascular diseases are a leading cause of death worldwide, but our understanding of the underlying mechanisms is limited, in part because of the complexity of the cellular machinery that controls the heart muscle contraction cycle. Cryogenic electron tomography (cryo-ET) provides a way to visualize diverse cellular machinery while preserving contextual information like subcellular localization and transient complex formation, but this approach has not been widely applied to the study of heart muscle cells (cardiomyocytes). Here, we deploy an optimized cryo-ET platform that enables cellular-structural biology in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Using this platform, we reconstructed sub-nanometer resolution structures of the human cardiac muscle th

SUBMITTER: Woldeyes RA 

PROVIDER: S-EPMC10634850 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature