Cell-type-resolved somatic mosaicism reveals clonal dynamics of the human forebrain.

ABSTRACT: Debate remains around anatomic origins of specific brain cell subtypes and lineage relationships within the human forebrain. Thus, direct observation in the mature human brain is critical for a complete understanding of the structural organization and cellular origins. Here, we utilize brain mosaic variation within specific cell types as distinct indicators for clonal dynamics, denoted as cell-type-specific Mosaic Variant Barcode Analysis. From four hemispheres from two different human neurotypical donors, we identified 287 and 780 mosaic variants (MVs), respectively that were used to deconvolve clonal dynamics. Clonal spread and allelic fractions within the brain reveal that local hippocampal excitatory neurons are more lineage-restricted compared with resident neocortical excitatory neur

SUBMITTER: Chung C 

PROVIDER: S-EPMC10634852 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature