Lack of chromokinesin Klp-19 creates a more rigid midzone and affects force transmission during anaphase in C. elegans.

ABSTRACT: Recent studies have highlighted the significance of the spindle midzone - the region positioned between chromosomes - in ensuring proper chromosome segregation. By combining advanced 3D electron tomography and cutting-edge light microscopy we have discovered a previously unknown role of the regulation of microtubule dynamics within the spindle midzone of C. elegans. Using Fluorescence recovery after photobleaching and a combination of second harmonic generation and two-photon fluorescence microscopy, we found that the length of the antiparallel microtubule overlap zone in the spindle midzone is constant throughout anaphase, and independent of cortical pulling forces as well as the presence of the microtubule bundling protein SPD-1. Further investigations of SPD-1 and the chromokines

SUBMITTER: Zimyanin V 

PROVIDER: S-EPMC10634869 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature